| Congresso Brasileiro de Microbiologia 2023 | Resumo: 1302-2 | ||||
Resumo:The use of microbial pigments in textile dyeing can add antimicrobial and antiviral properties to the dyed fabric. In addition, biopigments are a more sustainable alternative to use of synthetic dyes and contribute to reduce the environmental impacts of textile industry, moreover, providing alternative and sustainable technology development to minimize environmental impacts and conserve water. Janthinobacterium lividum is one of the most important bacteria known for producing violacein, an intense violet pigment with significant applicability in textile dyeing, particularly on silk fabric. The objective of this study was to evaluate the effect of temperature and agitation on the growth and production of violacein. The strain of J. lividum was cultivated in nutrient broth medium for up to 96 hours under controlled agitation (low and high) and temperature (low and high). Every 24 hours, the culture was sampled and analyzed for violacein production and growth. Growth was assessed by measuring the optical density (OD) at 600 nm and 750 nm. Violacein was extracted with ethanol, and after centrifugation, the extract was analyzed using a spectrophotometer (at 575 nm). The statistical analysis used as a factorial design (2 x 2) was conducted in the Jamovi program. The bacteria reached the stationary phase after 72 hours of growth when violacein production peaked. Violacein production was observed to be higher with increased agitation and temperature. Under these conditions, the bacteria initiated violacein production more rapidly and reached maximum production at 72 hours of growth, which did not significantly differ from that obtained at 96 hours. The fermentation conditions established in this study will enable the development of more efficient violacein production processes for J. lividum, benefiting its application in the textile industry and other sectors. Palavras-chave: Dyeing, Sustainability, Silk, Microbial pigments Agência de fomento:Conselho Nacional de Desenvolvimento Científico e Tecnológico; SETI-PR – Seda Brasil Project. | |||||